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Rapid dna dephos & ligation kits;

DNA with either blunt or sticky ends (≤200 ng). Heat inactivation 10 min at 65 °C (T4 Ligase) Inactivation of T4 Ligase is necessary only if the ligation reaction mixture is to be used in experiments other than transformation assays. Note Heat inactivation of the ligation reaction mixture before transformation causes the number of transformed colonies to decrease drastically (> factor of 20). Star activity Star activity should also be avoided. When double digests are performed with enzymes prone to star activity, consult the Roche Restriction Enzyme Poster (or the Roche Applied Science Catalog) to select the optimal buffer for a given enzyme combination. Whenever possible use Buffer H for double digests. Application The Rapid DNA Ligation Kit covalently joins either stickyended or bluntended DNA fragments, for example, for Cloning into plasmid or phage vectors. Adding linkers to plasmids or other DNAs. Recircularizing linear DNA. Features and Benefits